DEVELOPMENT AND APPROBATION OF A TECHNIQUE OF REVEALING PARVOVIRAL ENTERITIS USING POLYMERASE CHAIN REACTION
DOI:
https://doi.org/10.54890/.v3i3.793Abstract
Resume. Development recently new approaches for indicating and identification of microorganisms associated with advances in of molecular genetics. Develop and approbation a test system for quantitative determination of the DNA of the pathogen parvovirus enteritis by polymerase chain reaction (PCR). Designed primer pairs for detecting serotypes parvovirus enteritis in dogs. The result of the work conditions have been optimized for the detection of primer annealing CPv (2a, 2b, 2c) by PCR diagnostics.
Keywords:
parvovirus enteritis of dogs, PCR, optimization and approbation of test systems, primers.References
1. Touratier L. A., propos d’une epizootie canine de gastro – enterite a parvovirus // Bull. Acad. Veter. –1979. –Vol. 52, №4. – P.605-609.
2. Ejimi H., Aimi K., Tagawa M. Leukocite transfusion to puppies with canine parvovirus infection // Bull. Nippon Veter. Zootechn. Coll. Tokyo. - 1980. –Vol. 332. №5. – P.103-107.
3. Parrish CR, O’Connell PH., Evermann JF. et al. Natural variation of canine parvovirus. Science. – 1985. 230:1046-1048.
4. Parrish CR., Have P., Foreyt WJ. et al. The global spread and replacement of canine parvovirus strains. J Gen Virol. - 1988; 69 (Pt 5):1111-1116.
5. Parrish CR., Aquadro CF., Strassheim ML. et al. Rapid antigenic-type replacement and DNA sequence evolution of canine parvovirus. J Virol. - 1991; 65:6544-6552.
6. Parrish, C. R., C. F. Aquadro, and L. E. Carmichael. Canine host range and a specific epitope map along with variant sequences in the capsid protein gene of canine parvovirus and related feline, mink, and raccoon parvoviruses. Virology. - 1988. 166: 293-307.
7. Hasonova L., Pavlik J. Economic impact of paratuberculosis in dairy cattle herds: a review. Veterinarni Medicina. – 2006. - P.193-211.
8. Fluit A.D. Visser M.R., Schmitz F-J. // Clin.Microbiol. Rev. - 2001. - Vol. 14. - P. 836-871.
9. Gurtler V., Stanisich V.A. // Microbiology. - 1996. -Vol. 142. - P. 3-16.
10. Mullis К., Falona F., Powledge T.M. et al, - 2004. –Vol. 94. - P. 156-158.