РАЗРАБОТКА УНИВЕРСАЛЬНОЙ ИФА ТЕСТ СИСТЕМЫ ДЛЯ ВЫЯВЛЕНИЯ  АНТИТЕЛ К ВОЗБУДИТЕЛЮ ЧУМЫ

B.B. Baymurzinov; P.N. Deryabin; Т-S. Ponomareva; D.A. Adambekov

doi:10.54890/.v1i1.505

B.B. Baymurzinov P.N. Deryabin Т-S. Ponomareva D.A. Adambekov

DOI:

https://doi.org/10.54890/.v1i1.505

Abstract

Using Y.pestis capsular antigen and conjugate based on protein A Staphylococcus, labeled with horseradish peroxidase highly sensitive and specific IEAtest system for the detection o f antibodies to capsular antigen is developed. In contrast to the EIA test system with use of a conjugate based on antibodies againsthuman IgG EIAtest there is developed universal system and it detects antibodies, not only in humans but also in different animals. The sensitivity of this EIA test system is higher than the specificity of the reaction of a straight line o f gems agglutinations. In the study of immune sera against the 5 other bacteria there is shown its specificity. A test system was stable for 6 months after production (observation period) and can be used to assess the post-vaccination immunity in humans and camels immunized against plague, as well as to assess the dynamics o f antibody response in model experiments on immunization and infection of laboratory animals and evaluation seropositive animals in the monitoring of natural foci o f plague.

Keywords:

plague, capsular antigen F1, enzyme immunoassay, protein-based conjugate labeled with horse radish peroxidase.

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