DETERMINATION OF ANTIBIOTIC SUSCEPTIBILITY OF BACTERIA ISOLATED FROM WASTEWATER IN THE CITY OF BISHKEK (APRIL – JUNE 2025)
DOI:
https://doi.org/10.54890/1694-8882-2025-3-38Abstract
The aim of this study was to conduct a comprehensive assessment of the microbiological contamination of wastewater in Bishkek city and to determine the antibiotic susceptibility of isolated microorganisms. Between April and June 2025, five monitoring points were examined, including the wastewater discharge area near the thermal power plant (TPP), several sites along the Big Chui Canal (BCC) in different parts of the city, and the municipal wastewater treatment facilities. A total of 15 water samples were collected and analyzed using the titration method in accordance with the standards of the European Committee on Antimicrobial Susceptibility Testing (EUCAST).
The bacteriological analysis identified representatives of conditionally pathogenic microflora — Escherichia coli, Proteus mirabilis, and Enterobacter aerogenes. All isolated strains showed susceptibility to the tested antibiotics, including ciprofloxacin, ceftriaxone, imipenem, and amoxicillin/clavulanic acid. The highest level of microbial contamination was recorded near the TPP discharge area, likely due to high concentrations of organic matter and elevated temperature. Although no resistant strains were detected, the findings indicate a potential risk of antibiotic resistance emergence under the influence of climate-related factors such as rising temperatures and altered hydrological conditions. The study emphasizes the importance of regular microbiological and climate monitoring of Bishkek’s water bodies, improvement of wastewater control systems, and the development of preventive measures to curb the spread of resistant microorganisms in the context of climate change.
Keywords:
wastewater, microbiological analysis, antibiotic susceptibility, Escherichia coli, water safety, climate, BishkekReferences
1. Centers for Disease Control and Prevention (CDC). Antibiotic Use in the United States, 2021 Update: Progress and Opportunities. 2021. Available from: https://www.cdc.gov/antibiotic-use/pdfs/stewardship-report-2021-H.pdf. Accessed October 5, 2022.
2. Sanchez GV, Kabbani S, Tsay SV, Bizune D, Hersh AL, Luciano A, et al. Antibiotic stewardship in outpatient telemedicine: adapting Centers for Disease Control and Prevention core elements to optimize antibiotic use. Telemed J E Health. 2024;30 (4):951-962. https://doi.org/10.1089/tmj.2023.0229
3. Wang Q, Wang X, Wang J, Ouyang P, Jin C, Wang R, et al. Phenotypic and genotypic characterization of carbapenem-resistant Enterobacteriaceae: a nationwide longitudinal CRE study in China (2012–2016). Clin Infect Dis. 2018;67(Suppl 2): S196–S205.
4. Tumbarello M, Sali M, Trecarichi EM, Leone F, Rossi M, Fiori B, et al. Bloodstream infections caused by extended-spectrum-β-lactamase-producing Escherichia coli: risk factors for inadequate initial antimicrobial therapy. Antimicrob Agents Chemother. 2008;52(9):3244–3252.
5. Patel G, Huprikar S, Factor SH, Jenkins SG, Calfee DP. Outcomes of carbapenem-resistant Klebsiella pneumoniaeinfection and the impact of antimicrobial and adjunctive therapies. Infect Control Hosp Epidemiol. 2008;29(12): 1099-1106.
6. World Health Organization (WHO). Water and sanitation: data and statistics. Copenhagen: WHO Regional Office for Europe; 2019. Available from: http://www.euro.who.int/en/health-topics/environment-and-health/water-and-sanitation /data-and-statistics
7. Hara D, Bello-Toledo H, Dominguez M, Sigarroa C, Fernandez P, Vergara L, et al. Antibiotic resistance of bacterial isolates from freshwater samples on Fildes Peninsula, King George Island, Antarctica. Sci Rep. 2020;10:3145.
https://doi.org/10.1038/s41598-020-60035-0
8. Singh S. Traditional methods of infection prevention and control in the post-antibiotic era: a perspective. J Sci Res. 2020;64:167–174. https://doi.org/10.37398/jsr.2020.640124
9. Ma Y, Li M, Wu M, Li Z, Liu H. Occurrence and regional distribution of 20 antibiotics in groundwater recharge periods. Sci Total Environ. 2015;518–519:498–506. https://doi.org/10.1016/j.scitotenv.2015.02.100
10. Amarasiri M, Sano D, Suzuki S. Understanding human health risks caused by antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARG) in water environments: current knowledge and questions to be answered. Crit Rev Environ
Sci Technol. 2020;50:2016–2059. https://doi.org/10.1080/10643389.2019.1692611
11. Amaya E, Reyes D, Paniagua M, Calderon S, Rashid MU, Colque P, et al. Antimicrobial resistance patterns of Escherichia coli isolates from different aquatic environments in León, Nicaragua. Clin Microbiol Infect. 2012;18:E347–E354. https://doi.org/10.1111/j.1469-0691.2012.03930.x
12. World Health Organization (WHO). Antimicrobial resistance. Geneva: WHO; 2021. Available from: https://www.who .int/news-room/fact-sheets/detail/antimicrobial-resistance
13. Alexander J, Hembach N, Schwartz T. Evaluation of antibiotic resistance dissemination by wastewater treatment plant effluents with different catchment areas in Germany. Sci Rep. 2020;10:1–9. https://doi.org/10.1038/s41598-020-65635-4
14. Serwecińska L. Antimicrobial pharmaceuticals and antibiotic-resistant bacteria in water. Water. 2020;12:3313.
https://doi.org/10.3390/w12123313
15. Tesfaye H, Alemayehu H, Desta AF, Eguale T. Antimicrobial susceptibility profile of selected Enterobacteriaceae in wastewater samples from health institutions, abattoirs, downstream rivers, and treatment plants in Addis Ababa, Ethiopia.
Antimicrob Resist Infect Control. 2019;8:134. https://doi.org/10.1186/s13756-019-0588-1
16. Michael CA, Dominey-Howes D, Labbate M. The antimicrobial resistance crisis: causes, consequences, and management strategies. Front Public Health. 2014;2:145. https://doi.org/10.3389/fpubh.2014.00145
17. Lee J, Jeong JH, Shin J, Jang HM, Kim S, Song MS, et al. Quantitative and qualitative changes in antibiotic resistance genes after passing through municipal wastewater treatment processes. Sci Total Environ. 2017;605–606:906–914.
https://doi.org/10.1016/j.scitotenv.2017.06.250
18. Каримов Т. Х. Экологическая и санитарно-гигиеническая безопасность источников водоснабжения Кыргызской Республики. Евразийский Союз Ученых. 2019;4-2(61):24-30.
19. Касымов О.Т. Аширалиева Д.О., Джемуратов К.А., Умаралиева Г.Б., Арзыгулова К.Ш. Изучение микробиологического состава воды открытых водоёмов и сточных вод города Бишкек и устойчивости бактерий к
противомикробным препаратам. Здравоохранения Кыргызстана. 2025;2:62-70. https://doi.org/10.51350/zdravkg 2025.2.6.7.62.70
20. Tan C, Li V, Zhang J, Zhou W, Chen J, Li Y, et al. Presence, spread, and elimination of antibiotic-resistant bacteria and resistance genes in an urban drinking water supply system: a review. Front Environ Sci Eng. 2019;13. https://doi.org/10. 1007/s11783-019-1120-9
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